Fig. 5.
Leukocytes in CNS of mice with EAE after treatment with PBS or poly I:C. a Flow cytometry analysis of leukocytes (CD45high) in mice with EAE. Infiltration was not affected by poly I:C (n = 5) at 6 h. b Images show hematoxylin and eosin staining of spinal cord sections from mice with EAE 1 day after PBS or poly I:C treatment (original magnification is 4×). Black boxes show higher magnification (20×) of selected areas with infiltrates. Scale bars 50 µm. c Levels of IL-17 and IFN-γ mRNA were elevated in CNS of mice with EAE, but were not affected by poly I:C treatment at indicated times. ND not detected. d Levels of IL-10 message in CNS were significantly increased at 18 h in response to poly I:C. Number of mice was between 4 and 5 in each group