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. 2015 May 17;181(1):51–64. doi: 10.1111/cei.12621

Figure 2.

Figure 2

Bone marrow dendritic cells (BMDCs) from thymic stromal lymphopoietin (TSLP) knock-out (KO) and wild-type (WT) mice show no differences in cytokine secretion or maturation marker expression. (a) TSLP mRNA expression in WT BMDCs which were left unstimulated or which were stimulated overnight with TSLP, tumour necrosis factor (TNF)-α, CD40L or lipopolysaccharide (LPS) [mean ± standard error of the mean (s.e.m.)]. (b) Cytometric bead array (CBA) of supernatants from LPS-stimulated TSLP KO (o) and TSLP WT (▪) BMDCs (mean ± s.e.m.). (c) Fluorescence activated cell sorter (FACS) analysis of TSLP KO and WT BMDCs, which were left either unstimulated or were matured with TNF-α or LPS. GMFI = geometric mean fluorescence intensity. The bar charts represent the mean ± s.e.m. (a, b) TSLP KO n = 3, TSLP WT n = 3. (c) TSLP KO n = 3, TSLP WT n = 5. All data are representative of four independent experiments.