. 2015 May 10;181(1):87–99. doi: 10.1111/cei.12630

Table 1.

Primer pairs used to detect expression of target genes by real-time reverse transcription–polymerase chain reaction (RT–PCR)

Genes	Primer sequence (5′–3′)	Amplicon size(bp)	Annealing temp(°C)
TAT-eGFP	For: AAGGATCCTATGGCAGGAAGAAGCGGAGAC	808	56
TAT-eGFP	Rev: AGCGACGAAGAGAGCTCATGGTGAGCAAGG	808	56
FoxP3	For: ATAAAGCTTCCCCCAACCCTAGGC	1290	56
FoxP3	Rev: ATACTCGAGTTAAGGGCAGGGATTG	1290	56
CTLA-4	For: GTCAAGGGAACATTAGGG	349	58
CTLA-4	Rev: ATTCAAACCACCAGCAAA	349	58
IL-2	For: CTTGCTAATCACTCCTCACAG	451	57
IL-2	Rev: GTGTTGTCAGCCCTT	451	57
IFN-γ	For: AAGCGTCATTGAATCACACC	202	58
IFN-γ	Rev: CGAATCAGCAGCGACTCCTTAG	202	58
IL-10	For: ATCGATTTCTCCCCTGTG	318	58
IL-10	Rev: AATGGGAACTGAGGTATCAG	318	58
TGF-β	For: AAATCAACGGGATCAGC	332	57
TGF-β	Rev: TTGGTTGTAGAGGGCAAG	332	57
β-actin	For: TGGAATCCTGTGGCATCCATGAAAC	349	58
β-actin	Rev: TAAAACGCAGCTCAGTAACAGTCCG	349	58

Tat-eGFP = transactivating transcriptional factor-enhanced green fluorescent protein; FoxP3 = forkhead box protein 3; CTLA-4 = cytotoxic T lymphocyte 4; IL = interleukin; IFN = interferon; TGF = transforming growth factor; For = forward; Rev = reverse.