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. 2015 May 17;181(1):164–178. doi: 10.1111/cei.12620

Figure 3.

Figure 3

Interleukin (IL)-10RA down-regulates a pro-apoptotic protein, Bcl-2-like protein 11 (BIM), via an extracellular-regulated kinase (ERK)-dependent pathway in dendritic cells (DCs). DCs were transfected with small interfering RNA (siRNA) targeting green fluorescent protein (GFP) or IL-10RA. (a) Western blot analysis for expression of protein kinase B (AKT), p-AKT, ERK, pERK, p38, pp38 and BIM in DCs transfected with the siRNA. Equal amounts of protein were loaded and separated by sodium dodecyl sulphate-polymerase gel electrophoresis (SDS-PAGE). Western blot analysis was performed with the cell lysate and the identical monoclonal antibodies. β-actin was used as a loading control. (b) Western blot analysis of BIM in lysates of DCs transfected with siIL-10RA after overnight treatment with various kinase inhibitors such as an ERK inhibitor PD98059, an AKT inhibitor API2 and a p38 inhibitor SB203580. (c). Western blot analysis of BIM in lysates of DCs transfected with siGFP or siIL-10RA after 4 h of treatment with MG132 (a ubiquitin inhibitor). The data presented in this figure are representative of three independent experiments.