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. Author manuscript; available in PMC: 2015 Jun 16.
Published in final edited form as: J Immunol. 2006 Sep 1;177(5):3045–3054. doi: 10.4049/jimmunol.177.5.3045

FIGURE 1.

FIGURE 1

Localization of nCT, dmCT E112K/KDEV, dmCT E112K/KDGL, and mCT E112K in T84 cells after incubation. Cells were fixed and analyzed 4 h after the start of Alexa Fluor 488-conjugated nCT, dmCT E112K/KDEV, dmCT E112K/KDGL, and mCT E112K uptake. To identify the intracellular destination, colocalization with ER-Tracker Blue-White p-xylene-bispyridinium bromide, a marker for the ER, is shown in quasi-red (A), whereas BODIPY TR-cer-amide, a marker for the Golgi apparatus, is shown in red (B). The merged signals appear yellow. Typical image pictures are presented for each group. The yellow color area of nCT (■), mCT E112K (▥), dmCT E112K/KDEV (▨), and dmCT E112K/KDGL (Inline graphic) were quantified with ImageJ software (C). The values represent the mean ± 1 SEM for six pictures from two different experiments. Each picture covers 12.96 mm2 of culture surface, which was equal to 262,144 pixels. *, p < 0.05; **, p < 0.01, when compared with nCT; †, p < 0.05; ††, p < 0.01, when compared with mCT E112K.

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