. Author manuscript; available in PMC: 2015 Jun 16.

Published in final edited form as: J Immunol. 2006 Sep 1;177(5):3045–3054. doi: 10.4049/jimmunol.177.5.3045

Table II.

Comparison of the distribution of dmCTs and nCT in olfactory tissues

	CNS Tissues
	ON/E^a		OBs^a		ON/E^b	OBs^b
Nasal Adjuvants	5 μg	0.5 μg	5 μg	0.5 μg	5 μg	5 μg
nCT	9.61 ± 0.49^†c	4.03 ± 0.41	1.84 ± 0.55	0.26 ± 0.09	2.23 ± 0.07	1.36 ± 0.02
mCT E112K	3.97 ± 0.37*	4.01 ± 0.65	0.83 ± 0.16	0.12 ± 0.07	1.83 ± 0.19	0.19 ± 0.01**
E112K/KDEV	17.92 ± 1.59*^†	4.51 ± 0.58	<0.1**^†	<0.1	0.46 ± 0.14**^†	<0.1**
E112K/KDGL	14.79 ± 1.07*^†	4.73 ± 0.37	0.25 ± 0.03**^†	<0.1	0.39 ± 0.01**^†	<0.1**

Distribution of acridinium-labeled enterotoxins into the OBs and ON/E was determined 24 h after nasal application of 5 or 0.5 fig of acridinium-labeled nCT, mCT E112K, dmCT E112K/KDEV, or dmCT E112K/KDGL. The OB and ON/E were collected and analyzed for the presence of acridinium-labeled enterotoxin.

Groups of mice were nasally immunized with 100 fig of OVA plus 5 fig of acridinium-labeled nCT, mCT E112K, dmCT E112K/KDEV, or dmCT E112K/KDGL three times at weekly intervals. The distribution of acridinium-labeled enterotoxins in the OBs and ON/E were determined 7 days after the last nasal application. The OBs and ON/E were collected and analyzed for the presence of acridinium-labeled enterotoxin.

Data are expressed as nanograms per 10 mg of tissue ± SEM for nine mice in each experimental group. The CNS tissues of nine mice given nasal PBS were examined for background levels of luminescence. These control values were subtracted from each experimental value. Difference from the value of nCT was statistically significant. (*, p < 0.05; **, p < 0.01). †, P < 0.05, when compared with mCT E112K.