Skip to main content
. Author manuscript; available in PMC: 2015 Jun 16.
Published in final edited form as: Oncogene. 2012 Jul 9;32(21):2631–2639. doi: 10.1038/onc.2012.286

Figure 6. SIM2s inhibits metastasis and alters angiogenesis.

Figure 6

A – Immunohistochemical analysis of lung tissue for vimentin (VIM) positive micrometastases showed decreased staining in SIM2s tumors, whereas loss of SIM2s enhanced lung metastasis. Images were taken with a 40x objective (25.2x). B – Q-PCR analysis for human β-2-Globulin as an indicator of lung metastasis confirmed the effect of SIM2s on metastasis with a decreased in β-2-Globulin expression in SIM2s tumors and increased expression with loss of SIM2s. Data is shown as the number of β-2G positive samples out of the total number of samples analyzed. C – Increased magnification (63x) of VIM staining to indicate the presence of vimentin positive cells in Scrambled controls and SIM2si tumors. D – Immunostaining for CD31 expression showed that SIM2s tumors have smaller blood vessels that remained on the outer perimeter of the tumors, whereas SIM2si tumors had an increased trend in angiogenesis. E – Quantification of CD31 staining by measuring blood vessel length confirms trends seen with CD31 immunohistochemistry. Images were taken using a 10x objective (6.3x) and a 40x objective (25.2x). Scale bars in images represent 100μm. *= p-value < .05