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. Author manuscript; available in PMC: 2015 Jun 16.
Published in final edited form as: Parasite Immunol. 1998 Oct;20(10):455–462. doi: 10.1046/j.1365-3024.1998.00174.x

Figure 1.

Figure 1

IL-12 modulation of cytokine production in lungs and spleen. C57Bl/6 mice were immunized × 3 s.c. with 100 000 killed B. malayi larvae (microfilariae) and injected intravenously with 200 000 live parasites. One group of animals received either saline or IL-12 i.p. during the week of initial immunization with parasite antigens. Ten days after intravenous injection of parasite larvae, lungs were processed for RT-PCR, and splenic lymphoid cells were stimulated with parasite antigens. (a): Densitometric representation of cytokine gene expression in the lungs of control and IL-12 treated animals after RT-PCR and Southern transfer. The expression of each cytokine was calculated as a ratio of the cytokine band intensity to the intensity of the housekeeping gene HPRT (OD ratio) (b): Spleen cells were incubated with parasite antigen (10 μg/ml) for 72 h, and cytokines released into the supernatant were measured by two-site ELISA. Note the increased IFN-γ and decreased IL-4 and IL-5 production in both tissues of IL-12 treated animals. Data are mean ± SD of five animals per group, and are representative of four repeat experiments. Asterisks represent statistical significance between control and IL-12 treated animals (P > 0·05).