Fig. 7.
Activity of GAS alphamer in opsonophagocytic killing assays and whole blood killing assay. a Late exponential phase GAS SF370 was pre-incubated with GAS alphamer α20A24P or control alphamer αRAND-80. After 20 min, neutrophils and human IgG (hIVIG) were added. Samples to which vehicle (HBSS+/+) and hIVIG instead of neutrophils were added served as no phagocyte controls. At various time points, the percentage of live colony forming units as compared to time point 0 min was determined. Quadruplicate to hexuplicate samples were run for each condition, and average bacterial percentage values as compared to the initial bacterial concentrations±SD plotted. *p<0.05; ***p<0.001, α20A24P vs. αRAND-80 in presence of neutrophils; two-way ANOVA with Bonferroni post test. b 1.1×105 CFU/mL of GAS M1T1 5448 were pre-incubated for 15 min with 1 μM of the GAS alphamer α20A24P (closed bars) or the control alphamer αRAND-80 (open bars) in a total volume of 30 μL. Then, 120 μL of human blood was added. Thus, the alphamer end concentrations were 200 nM. After 60, 120, 180, and 240 min of co-incubation, the bacterial concentrations were determined. Each sample was run in quadruplicate and average values± SD are plotted. *p<0.05, α20A24P vs. αRAND-80 two-way ANOVA with Bonferroni post tests