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RACPP schematic showing (A) no tumor-contrast immediately post-injection; (B) high tumor-contrast following MMP-dependent cleavage, separating Cy5 from Cy7. (C) Application of RACPP to HNSCC specimens produces faster Cy5/Cy7 ratio change compared to normal tissue. Abbreviations: RACPP, ratiometric activatable cell-penetrating peptide; HNSCC, head and neck squamous cell carcinoma.
