Fig. 2.
CD8 T cell IFN-γ is increased in mice that receive Cy and lysate/CpG. a Groups of mice were treated (no treatment, Cy only, Cy and CpG, Cy and lysate or Cy and lysate/CpG) according to the schedule. On day 22, spleens from 3 mice per group were harvested and pooled. b CD8 T cells were purified by immunomagnetic cell separation and 105 were incubated overnight with 105 AgN2a stimulator cells. IFN-γ production was analyzed per ELISPOT assay. Background signal (T cells only with no tumor cell stimulators) was subtracted from the experimental values. The graph represents data from 1 to 2 independent experiments. Data was analyzed for statistical significance using one-way ANOVA