Fig. 3.

Effects of REG4 on the invasive and metastatic ability of ovarian cancer cells. The treatment with the REG4-expressing plasmid or the recombinant protein induces migration in wound healing assays (a, n = 3), and invasion in Matrigel transwell assays (b, n = 3) compared with the control and mock cells. In addition, expression of Wnt5a, p70s6k, survivin and VEGF is increased at both mRNA and protein levels in SKOV3, as observed by (c) real-time RT-PCR and (d) Western blotting, respectively; however, Bax expression was decreased. Results are expressed as mean ± SD of three separate experiments; *P < 0.05