A. Temporal mapping of mean percentage of cells exhibiting calcium activity in vehicle LRO (n=29 embryos) from bud-stage to 16-somite-stage. Calcium activity in the LRO was categorized as ICOs (green), cilia-to-cytosolic waves (magenta) and cytosolic waves (cyan). B. Spatiotemporal mapping of mean percentage of cells per embryo exhibiting ICOs on the left or right-side of the LRO in vehicle embryos (n=10 embryos) during bud, 1–4 somite, 5–9 somite and 10–16 somite stages. Left-sided calcium oscillations = blue; right-sided calcium oscillations = orange. C–D. Spatiotemporal mapping of two cilia populations in the zebrafish LRO. (C) Temporal mapping of mean percentage of motile cilia in the vehicle (gray), pkd2 MO (green) and c21orf59 MO (violet) LROs over the course of LRO development. (D) Additive projections of motile (magenta) and immotile (cyan) cilia from vehicle embryos over the course of LRO development. Tailbud area and morphology (gray) of corresponding LROs are also assembled by additive projections. A: Anterior, P: Posterior, L: Left, R: Right. All data shown is mean±SEM. See also Figure S4.