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. Author manuscript; available in PMC: 2016 Mar 2.
Published in final edited form as: Curr Biol. 2015 Feb 5;25(5):556–567. doi: 10.1016/j.cub.2014.12.051

Figure 5. Intraciliary calcium is essential for vertebrate LR development.

Figure 5

A. Representative brightfield and fluorescent images showing heart position in zebrafish embryos at ~30-somite stage highlighted by a GFP transgene driven by the promoter of cardiac myosin light chain 2 (cmlc2). The embryo is seen from the ventral side, showing normal left (blue), and abnormal middle (red) and right (green) heart loops. Graph shows distribution of heart position in response to expression of parvalbumin (PVALB) and mutant parvalbumin (PVALB-CD) targeted to cilia or cytoplasm of wildtype embryos. Data shown is pooled from nine total independent experiments. B. Representative images of whole-mount in situ hybridization for dand5 (charon) expression in the LRO of zebrafish embryos at the 8–10 somite stage. The embryo is seen from the dorsal side, showing normal right-sided (blue) expression and abnormal bilateral (red), left-sided (green) or absent (orange) expression. Graph shows distribution of dand5 in response to expression of PVALB and mutant parvalbumin (PVALB-CD) targeted to cilia or cytoplasm. Data shown is pooled from three independent experiments. Statistical comparison was analyzed by one-way ANOVA with Tukey’s multiple comparison test. *P < 0.05, while ** P < 0.005, ***P < 0.0005, respectively. NS (not significant): P ≥ 0.05. Asterisks above brackets denote comparisons between samples indicated by the bracket, while asterisks above a single sample denote comparisons between that sample and the control. n = Total number of embryos analyzed for each experimental condition (in parentheses). See also Figure S6.