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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Methods Mol Biol. 2015;1309:151–169. doi: 10.1007/978-1-4939-2697-8_13

Fig. 5.

Fig. 5

A laser-scanning confocal micrograph of the fluorescent signal from the periphery of an oocyte RNA 7 days after injection with MscS-GFP cRNA. An oocyte in ND96 buffer was placed onto a glass slide with concaved bottom and covered with a coverslip. Under bright field the lens was focused on the edge of the oocyte, then the confocal scan with GFP filters (488/510 nm) setup was performed. Inset: oocyte image in bright field