Figure 5.

Role of VEGFR1 in Cancer Pain following Hind Paw Implantation of C57BL6-Isogenic Lewis Lung Carcinoma Cells

(A) Western blot analysis on DRGs from SNS-Vegfr1^−/− and Vegfr1^fl/fl (control) mice.

(B–G) Analysis of SNS-Vegfr1^−/− and Vegfr1^fl/fl mice (n = 6–8 mice/group) with respect to intraplantar VEGF-A-induced mechanical (B) and thermal hypersensitivity (C), tumor-associated hypersensitivity (D), tumor-induced hypertrophy and sprouting of PGP-9.5-immunoreactive sensory nerves (E and F), and tumor growth (G), including negative control for immunostaining and an H&E-stained section.

(H and I) Tumor-induced mechanical hypersensitivity (H) and tumor growth (I) in VEGFR1-Tk^−/− and WT mice.

^∗p < 0.05 as compared with sham in (F) and compared with basal in other panels, ANOVA followed post hoc Fisher’s test; in (D) and (H), ANOVA for repeated-measures was performed; ^†p < 0.05 as compared with corresponding control group, ANOVA for random measures followed by post hoc Fisher’s test. Scale bar represents 50 μm in (E). Data are presented as mean ± SEM.