Fig. 2.

Similar plasma cytokine concentrations, lipoprotein profile, and atherosclerotic lesion size of ApoE^−/− and TCRδ^−/−ApoE^−/− mice. (A) Plasma cytokine concentrations were similar in ApoE^−/− (closed bar) and TCRδ^−/−ApoE^−/− (open bar) mice within the same diet group. Plasma cytokine were quantified with MSD multiplex assay with fasting plasma samples collected from mice fed a chow or 10 weeks of western diet. (B) Plasma cholesterol and lipoprotein fractions were the same in of ApoE^−/− and TCRδ^−/−ApoE^−/− mice. Age- and gender-matched ApoE^−/− and TCRδ^−/−ApoE^−/− mice were fed Western diet for 10 weeks. Fasting plasma were collected, and plasma lipids and lipoproteins were analyzed by FPLC. Each sample for FPLC analysis were pooled from plasma samples from 5 mice. Results are shown in mean ± SEM. N = 5 per group. (C) No significant difference in the development of atherosclerosis in TCRδ^−/−ApoE^−/− mice. Left: Quantification of plaque area as % of aortic surface in matched ApoE^−/− and TCRδ^−/−ApoE^−/− mice after 10 weeks of Western diet feeding. Right: Representative Oil Red O staining (red) in aortic ApoE^−/− and TCRδ^−/−ApoE^−/− mice after 10 weeks of Western diet feeding. Results are shown in individual dots and mean ± SEM. N = 12–14 per group. *P < 0.05, **P < 0.01, ns: not statistically significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)