Skip to main content
. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Nat Struct Mol Biol. 2015 Mar 16;22(4):298–303. doi: 10.1038/nsmb.2985

Figure 3.

Figure 3

Unique residues of Pol ν for mutagenic synthesis. (a) Sequence alignment of helices O and Oa. Generally conserved residues are colored blue. Uniquely conserved residues are outlined in orange for stabilizing the open-state of Pol ν, and in green for dT misincorporation. (b) E675 and K679 and corresponding residues in Pol I. (c,d) Nucleotide incorporation in the mutation hotspot (c) and (d) coldspot by Klenow, WT, E675R and K679A Pol ν75 with each (A, G, T or C) or all four dNTPs (4) provided. Kinetic data and the preference of dC over dT (ratios of kcat/KM for dC over dT) are shown below. (e) A TG mismatch is stabilized by Lys or Arg in the major groove.