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. Author manuscript; available in PMC: 2015 Jun 17.
Published in final edited form as: Bone. 2010 Sep 18;48(2):406–413. doi: 10.1016/j.bone.2010.09.007

Figure 4.

Figure 4

The primary human mesenchymal stem cells (BMSC and ADAS) stimulated with rrAmbn added as a single pulse to the medium showed a rapid, ten-fold increase in AMBN expression peaking after three hours before steadily declining towards background levels. After 12 hours the AMBN expression in the stimulated cells was only about two times that of the unstimulated cells and after 24 hours the stimulated AMBN mRNA expression levels had completely returned to the level of the controls. In unstimulated cells the AMBN mRNA expression remained stable at a level of about 2% of the housekeeping genes throughout the observation period. The values are mean of two real time RT-PCR reactions run on pooled triplets of cell culture samples (n=6). Error bars are not included in this panel for better visualization, but the values at 3 and 12 hours were statistically significant (p=0.029). At 24 hours the difference between stimulated and unstimulated samples was not statistically significant (p>0.05) when using paired samples and Mann-Whitney Rank Sum Test.