Table 1.
Primers used in real-time RT-PCR analysis of ameloblastin mRNA expression.
| Gene | Primer sequence | Species | Amplicon size (bp) |
|---|---|---|---|
| AMBN | S 5’ - AGCCATGTTTCCAGGATTTG - 3’ A 5’ - TGCACCTCCTTCTTCGTTCT - 3’ |
Human | 142 |
| Ambn | S 5’ - GCGTTTCCAAGAGCCCTGATAAC - 3’ A 5’ - AAGAAGCAGTGTCACATTTCCTGG - 3’ |
Mouse | 366 |
| GAPDH | S 5’-TGCACCACCAACTGCTTAGC - 3’ A 5’-GGCATGGACTGTGGTCATGAG - 3′ |
Human | 87 |
| GAPDH | S 5’-ACCCAGAAGACTGTGGATGG- 3’ A 5’-CACATTGGGGGTAGGAACAC - 3` |
Mouse | 171 |
| Beta-Actin | S 5’ - CTGGAACGGTGAAGGTGACA- 3’ A 5’ - AAGGGACTTCCTGTAACAATGCA - 3’ |
Human | 140 |
| Beta-Actin | S 5’ - GCTTCTTTGCAGCTCCTTCGT- 3’ A 5’ - ATATCGTCATCCATGGCGAAC - 3’ |
Mouse | 64 |