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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Dev Biol. 2015 Apr 24;403(1):30–42. doi: 10.1016/j.ydbio.2015.03.017

Figure 5. Macrophage engulfment of embryonic endothelial cell membrane particles is not due to apoptosis.

Figure 5

At E16.5, there is no indication of Flk1-H2B::YFP+ apoptotic nuclear fragments or co-localization with Flk1-myr::mCherry+ membrane particles within the macrophages (arrowheads in A-C). By the time EC apoptosis is known to occur (postnatal day 7), activated Caspase 3+ staining is observed as co-localizing with YFP+ nuclear fragments thereby validating Flk1-H2B::YFP+ as a readout for apoptosis (D-F). N>5.