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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Dev Biol. 2015 May 6;404(1):1–13. doi: 10.1016/j.ydbio.2015.04.023

Figure 7. Ovarian muscle sheaths provide structural support during oogenesis, which affects female fertility.

Figure 7

Stainings of adult ovaries by rhodamine phalloidin (A,B,I,J green) or anti-α-Spectrin (C,E,G, white; H,J red), anti-FasIII (D,F, red), and anti-Vasa (D,F,H, green) antibodies.

(A,B) The role of htl in morphogenesis of ovarian muscles was examined using c587.GAL4 somatic cell driver: control, driver alone (A) compared with htl KD (using UAS.htl.RNAi40627, B). A white arrow and arrowhead in (B) mark absence and aberrant organization of ovarian muscle sheaths, respectively.

(C-H) The role of htl in structural organization of adult ovarioles was investigated using the somatic cell c587.GAL4 driver: control, driver alone (C,D) compared with htl KDs [using UAS.htl.RNAi6692 (E,F) and using UAS.htl.RNAi40627 (G,H)]. Yellow arrows in (E-H) mark “flop-down” egg chamber phenotypes. Phenotypes for two time points are shown: two days old (2OD; C,E,G) and ten days old (10OD: D,F,H).

(I,J) Timing of htl action was examined by using GAL80ts to limit htl KD to the adult stage. htl.GAL4GMR93H07 driver together with GAL80ts were used to support expression of UAS.htl.RNAi40627 only in adults, by switch to growth at 29°C. In panels (A) and (C), “A” denotes the anterior tip and “P” denotes the posterior tip.