Fig. 6.

ROS generation, measured by DCFH-DA, in aortas obtained from rats treated with ethanol (1.5 g/kg) 30 min following AP-5 (5 mg/kg, i.v.) or its vehicle (saline). Aorta homogenates were incubated at 37 °C with DCFH for 30 min. Kinetic readings were recorded at 5-min intervals; relative fluorescence units were normalized to protein. Values are means ± SEM of 4–6 observations.