Table 1.
Sample | Insert size (bp) | Read length (bp) | Raw data (Gbp) | HQ data (Gbp) | ||
---|---|---|---|---|---|---|
Total data (Gbp) | Valid sequence depth (×) | Total data (Gbp) | Valid sequence depth (×) | |||
TM-1 | 180 and 300 | 2 × 100 | 185.0 | 74.0 | 154.7 | 61.9 |
Hai7124 | 300 | 2 × 100 | 111.8 | 44.7 | 97.9 | 39.1 |
THF2 a | 300 | 2 × 100 | 938.0 | 375.2 | 784.0 | 313.6 |
aTHF2: 59 F2 populations from a cross between TM-1 and Hai7124. The estimated genome size is 2.5G. The cutoff value for the PHRED quality score for high-quality filtering is 20. The cutoff value for the percentage of read length that should be of given quality is 70