Table 2.
PCR fragment sizes with defined primer combinations as calculated based on the chromosomal insertion*
| Forward Primer | Reverse Primer | Resulting PCR-Fragment length in base pairs (bp) |
|---|---|---|
| ALA2b | ALA3b | 88 |
| ALA2b | P2Bc | 290 |
| ALA2b | P2Dc | 941 |
| ALA2b | MP4b-F | 1516 |
| MP-3 F | DOW2.55 | 1156 |
| MP-3 F | DOW2.5 | 1718 |
| MP-3 F | ALA3b | 2078 |
| MP-3 F | P2Bc | 2280 |
| MP-3 F | P2Dc | 2931 |
| MP-3 F | MP4b-F | 3506 |
| P1Ac | P2Dc | 410 |
| P1Ac | MP4b-F | 985 |
| MF-2 F | MF-2R | 3435 |
*When the insertion is not present at the examined locus, no specific PCR fragments should be able to be generated, except for the last primer pair, which should then give a PCR product of 459 base pairs (bp)