Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 16;10(6):e0129309. doi: 10.1371/journal.pone.0129309

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

Fig 2 — Serum was adsorbed against protein lysates from the E. coli expression vector and His-tagged LacZ prior to western blotting. Recombinant AM854 (54kDa), AM936 (36kDa) and as a negative control, AM1096 (100kDa), were used as antigen. There is no antibody response to any of the proteins prior to immunization (Pre-immune). After immunization and adsorption (Adsorbed), anti-AM854 and anti-AM936 antibody, but no antibody directed toward AM1096 or the fusion tags was detected. One representative animal, 146, is shown. A serum dilution of 1:100 was used in all western blots. The anti-V5 antibody at a dilution of 1:500 demonstrates the presence of recombinant protein in each well.