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. 2015 Jun 18;5:37. doi: 10.1186/s13568-015-0117-4

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© Liang et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Phylogenetic tree of deduced amino acid sequences of alkylsuccinate synthetase genes (assA) genes from methanogenic alkanes-degrading enrichment culture (in red). Clone sequences with green were detected in the stage III by Wang et al. (2012). The topology of the tree was obtained by the maximum likelihood method. Bootstrap values (n = 1,000 replicates), values below 70% are not shown.