Figure 2.

H₂O₂ in PBS and MEM. a PBS and MEM or b compounds (10 μM) were incubated plus or minus 1 mM sodium pyruvate (1 mM) or 35 U catalase at 37°C in a 5% CO₂ incubator for 60 min in the presence of 25 μM H₂O₂ substrate. Measured luminescence is shown as relative light units (RLU) in part a. Measured luminescence was converted to concentration of H₂O₂ after background correction using a standard curve in part b. Data points are shown with error bars representing ± SEM. Data is representative of three separate experiments with triplicate determinations of each data point.