Fig. 3.

Food intake, BW gain, and meal patterns after intra-VTA administration of subthreshold doses of amylin (0.04 μg) or its vehicle (100 nl of aCSF) plus leptin (0.1 μg) or its vehicle (100 nl of 0.01 M sodium bicarbonate). A and C: although these doses of amylin and leptin do not impact 24-h food intake when delivered separately, the combination of the 2 peptides significantly reduces feeding (A) by suppressing meal size (C). D: no effect on meal number was observed. B: intra-VTA delivery of this dose combination of leptin and amylin also significantly reduces 24-h BW change. *P < 0.05 compared with Veh/Veh. All data are shown as means ± SE.