Figure 3.

Representative 2-DE images of proteins in various germination stages from E. arvense spores. (A) Mature spores. (B) Rehydrated spores. (C) Double-celled spores. (D) Germinated spores. (E) Spores with protonemal cells. Proteins were separated on 24 cm IPG strips (pH 4–7 linear gradient) using IEF in the first dimension, followed by 12.5% SDS-PAGE gels in the second dimension. The 2-DE gel was stained with Coomassie Brilliant Blue. A total of 80 differentially abundant proteins identified by ESI-Q-TOF and ESI-Q-Trap tandem mass spectrometry are marked with numbers on the gels. Molecular weight (MW) in kDa and pI of proteins are indicated on the left and top of the gels, respectively. Detailed information can be found in Table 1 and Supplementary Table S1.