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The regulation of ISGs by EPSTI1. Huh7.5 cells were transfected with p3XFLAG-CMV-EPSTI1 or vector followed by HCV infection (MOI = 0.1). The cells were harvested at 48 h after infection. IFI44L (a), ISG12a (b), 1-8U (c), ISG60 (d), PKR (e), and OAS1 (f) mRNA were detected by real-time PCR and normalized with GAPDH.
