Figure 2.

Identification of the regions for binding between katanin-p60 and USP47. A, Deletions of USP47 (UΔ1–UΔ5) were tagged with HisMax to their N termini and expressed in HEK293T cells with and without Flag-Kp60 (top). Cell lysates were subjected to pulldown with NTA resins followed by immunoblot with anti-Flag or anti-Xpress antibody (bottom). B, Deletions of katanin-p60 (KΔ1-KΔ4) were tagged with V5-His to their C termini and expressed in cells with and without Flag-USP47 (top). Cell lysates were subjected to pulldown with Flag-M2-beads followed by immunoblot with anti-V5 or anti-Flag antibody (bottom). In the top panels, whether the proteins bind to each of the deletions or not was shown as “+” or “−,” and the binding regions were indicated by the arrows. The single and double asterisks indicate IgG heavy and light chains, respectively.