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Ferristatin II induces hepcidin expression in vivo. Transcript levels of hepcidin, Smad 1/5/8 downstream targets (Smad7, BMP6, and Id1), and the acute-phase reactants α-2-macroglobulin (α2M), α-1-acid glycoprotein (AGP), and C-reactive protein (CRP) in rats treated with or without ferristatin II. The fold change in transcript level compared with control was calculated by the comparative ΔΔCt method with 36B4 as the reference gene; n = 4, *P < 0.01.
