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. 2015 Apr 23;308(12):G1019–G1026. doi: 10.1152/ajpgi.00324.2014

Fig. 2.

Fig. 2.

Ferristatin II does not induce endoplasmic reticulum (ER) stress in vivo or in vitro. A: XBP splicing is not induced in vivo. Levels of spliced, unspliced, and total XBP1 message in livers from rats treated with or without ferristatin II were determined by qPCR. Shown is the % spliced XBP1 (sXBP1) determined on the basis of total XBP1 and the fold difference in total determined for control and treated rats. B: Grp94 is not induced in vitro. HeLa cells were treated overnight with thapsigargin (1 μM) or tunicamycin (1 μg/ml) or treated 4 h with ferristatin II (50 μM). Cell lysates were immunoblotted to determine levels of Grp94. Actin was used as a loading control. Shown below are mean density ratios of Grp94/actin ± SE compared with vehicle controls (n = 3, *P < 0.05 and **P = 0.0001 determined by 2-tailed Student's t-test).