Table 1.

Baseline cardiac function in ex vivo perfused hearts isolated from NCD-fed or HFD-fed WT and mTOR-Tg mice

	WT NCD-Fed Group	mTOR-Tg NCD-Fed Group	WT HFD-Fed Group	mTOR-Tg HFD-Fed Group
Number of mice/group	24	28	24	26
LV systolic pressure, mmHg	133.6 ± 6.3	134.8 ± 4.2	163.7 ± 7.7**	160.0 ± 7.0**
LV end-diastolic pressure, mmHg	3.60 ± 0.43	4.83 ± 0.48	4.92 ± 0.43	4.94 ± 0.52
LV developed pressure, mmHg	130.0 ± 6.2	130.0 ± 4.2	158.8 ± 7.8**	154.9 ± 7.1**
LV dP/dtmax, mmHg/s	5,356 ± 314	5,476 ± 239	7,183 ± 325***	6,836 ± 380**
LV dP/dtmin, mmHg/s	−2,937 ± 141	−2,959 ± 107	−3,822 ± 107***	−3,621 ± 162**
Heart rate, beats/min	308.1 ± 8.1	299.5 ± 9.4	285.8 ± 14.7	277.5 ± 11.8
Coronary flow, ml/min	3.14 ± 0.22	3.19 ± 0.25	4.23 ± 0.18***	3.98 ± 0.20*
Heart weight, mg	139.67 ± 5.4	134.00 ± 3.9	161.92 ± 3.86**	150.65 ± 3.36**†
Heart weight/tibia length, mg/mm	6.23 ± 0.24	5.95 ± 0.16	7.23 ± 0.16**	6.78 ± 0.15***†

Values are means ± SE. WT, wild-type mice; NCD, normal chow diet; mTOR-Tg, mechanistic target of rapamycin-overexpressing transgenic mice; HFD, high-fat diet.

^*P < 0.05,

^**P < 0.01, and

^***P < 0.001, NCD vs. HFD;

^†P < 0.05, WT vs. mTOR-Tg groups (by Student’s t-test).