Fig. 7.

Effect of Rhcg deletion on the response to dietary protein restriction. Top: urinary ammonia excretion. Dietary protein restriction induced a rapid decrease in urinary ammonia in both control and global Rhcg deletion mice. There was no significant difference in urinary ammonia excretion between mice with global Rhcg deletion and those with intact Rhcg expression, either while receiving basal, 20%, protein diet or after initiation of 6% protein diet. Bottom: urinary pH. There was no significant difference in urinary pH between mice with global Rhcg deletion and those with intact Rhcg expression when fed 20% protein diet; however. in mice 6% protein diet, Rhcg deletion mice excreted more alkaline urine than Rhcg intact mice. *P < 0.05, 20 vs. 6% protein diet in wild-type (WT) mice; #P < 0.05, 20 vs. 6% protein diet in global Rhcg KO mice; $P < 0.05, WT vs. global Rhcg KO mice in 6% protein diet; n = 11/group on 20% protein and 6% protein diet on WT mice and n = 5/group on 20% protein and 6% protein diet on global Rhcg KO mice.