Fig. 1.

Experimental design. C57BL/6J dams were maintained on a control (4 mg/kg folic acid) or control diet supplemented with folic acid (24 mg/kg folic acid) for 2 wk before mating. Timed matings were performed, and the resulting male offspring were maintained on maternal diets through lactation, weaning, and adulthood. Body mass and food and water consumption were measured at weekly intervals starting at 3 wk of age and continuing through the end of the experiment at 14 wk of age. Six-week-old adult male mice underwent subcutaneous implantation of testosterone and estradiol (T+E2) pellets. Physiology and voiding function were determined 1 or 2 mo later at 10 and 14 wk of age, respectively.