A folic acid-enriched diet does not protect against T+E2 increases in bladder volume and prostate wet weight. Adult male mice were treated as described in Fig. 1. The following parameters were quantified at time of euthanasia in animals not undergoing cystometry: bladder volume (A), wet weights of anterior prostate (B), dorsal prostate (C), lateral prostate (D), ventral prostate (E), seminal vesicle (F), urethra (G), bladder (H), and relative lateral prostate (I). Lower urinary tract histology was examined in hematoxolin- and eosin-stained cross sections of urethra (J) and bladder (K). Values are means ± SE. *Significant difference, P ≤ 0.05; n ≥10/group.