Fig. 9.

A folic acid-enriched diet protects against a T+E2-induced increase in intervoid interval and bladder capacity and compliance. Adult male mice were treated as described in Fig. 1. At 2 mo postsurgery, urethane-anesthetized mice were analyzed by cystometry. A: representative cystometrograms from animals in each treatment group which elicited voiding contractions. Inset: representative of the 30% of T+E2 mice on the control diet which did not elicit a voiding contraction during the 2-h monitoring period and instead showed a steady rise in pressure with no apparent voiding contractions. The following parameters were quantified from the remainder of mice: intervoid interval (B), time back to baseline after peak voiding pressure (C), leak point pressure (D), threshold pressure to elicit a void (E), drip voids (1–2 drops) over a 6-sustained void (3 or more drops) interval (F), number of nonvoiding contractions over a 6-sustained void interval (G), peak-to-baseline pressure of voiding contractions (H), peak-to-baseline pressure of nonvoiding contractions (I), bladder capacity (J), and bladder compliance (K). Values are means ± SE. *Significant difference, P ≤ 0.05; n ≥5/group.