Table 1.

Properties of the fluorescent proteins (FPs) used in this study.

FP*	λem (nm)	λem (nm)	QY	EC	Brightness	Oligomerization	Reference	Codon
EBFP2	383	448	0.56	32000	18	Monomer	Ai et al. (2007)	n.a.
SBFP2	383	448	0.47	34000	16	Monomer	Kremers et al. (2007)	n.a.
TagBFP	402	457	0.63	52000	32.8	Monomer	Subach et al. (2008)	Mammal
mCerulean	433	475	0.62	43000	26.7	Monomer	Rizzo et al. (2004)	n.a.
TagCFP	458	480	0.57	37000	21	Monomer	Evrogen	Mammal
TagGFP2	483	506	0.6	56500	33.9	Monomer	Evrogen	Mammal
sfGFP	485	510	0.65	83300	54.1	Monomer	Pedelacq et al. (2006)	Bacteria
TagYFP	508	524	0.62	50000	31	Monomer	Evrogen	Mammal
mYPet	517	530	0.77	104000	80.1	Monomer	Nguyen and Daugherty (2005)	n.a.
mKO2	551	565	0.62	63800	39.6	Monomer	Lee et al. (2013)	Yeast
TagRFP-T	555	584	0.41	81000	33.2	Monomer	Shaner et al. (2008)	Mammal
mCherry	587	610	0.22	72000	15.8	Monomer	Shaner et al. (2004)	n.a.
mKate2	588	633	0.4	62500	25	Monomer	Shcherbo et al. (2009)	Mammal
eqFP670	605	670	0.06	15700	0.9	Dimer	Shcherbo et al. (2010)	Mammal
TagRFP657	611	657	0.1	34000	3.4	Monomer	Morozova et al. (2010)	Bacteria

*The properties are: λ_em/λ_em, wavelengths for excitation/emission maxima; QE, quantum yield; EC, extinction coefficient; brightness are calculated from QE and EC; reported oligomerization property; and codon usage for the versions used in this study.