Table 2.
Plasmids used in this study.
| Plasmids | Descriptions | Reference |
|---|---|---|
| pKD13 | aph frt (AmpR) | Datsenko and Wanner (2000) |
| pKD46 | Para::gam bet exo (AmpR) | Datsenko and Wanner (2000) |
| pCP20 | Pr-flp (AmpR CmR) | Datsenko and Wanner (2000) |
| pDonR P4-P1R | Gateway plasmid entry 1 | Invitrogen |
| pDonR 211 | Gateway plasmid entry 2 | Invitrogen |
| pDonR P2R-P3 | Gateway plasmid entry 3 | Invitrogen |
| pDEST R4-R3 | Gateway destination vector | Invitrogen |
| pTagBFP-C1 | Pcmv::TagBFP (KanR) | Evrogen |
| pTagCFP-C1 | Pcmv::TagCFP (KanR) | Evrogen |
| pTagYFP-C1 | Pcmv::TagYFP (KanR) | Evrogen |
| pTagGFP2-C1 | Pcmv::TagGFP2 (KanR) | Evrogen |
| pmKate2-C1 | Pcmv::mKate2 (KanR) | Evrogen |
| pTagRFP-T | PT7::tagRFP-T (AmpR) | Shaner et al. (2008) |
| pNirFP-N1 | Pcmv::eqFP670 (KanR) | Evrogen |
| pBAD-EBFP2 | Para::ebfp2-6xhis (AmpR) | Addgene, Michael Davidson |
| pSBFP2-C1 | Pcmv::sbfp2 (AmpR) | Kremers et al. (2007) |
| pyomKO2 | pFA6a-link-yomKO2 (KanR) | Lee et al. (2013) |
| pFX40 | Plac::yfp-minD minE (AmpR) | Shih et al. (2002) |
| pKD3ftsQAZ | PftsQAZ (AmpR) | Dai and Lutkenhaus (1991) |
| pFB174 | Para::mreBCD (CmR) | Bendezú and de Boer (2008) |
| pBVS3 | Plac::yfp-minD minE::aph frt (KanR, AmpR) | Wu et al. (2015) |
| pBVS4 | Plac::sfgfp-minD minE::aph frt (KanR, AmpR) | Wu et al. (2015) |
| pERA001 | Gateway destination vector with Prha (TetR) | This work |
| pERB006 | pERA001::hupA-ebfp2::aph frt (KanR, AmpR) | This work |
| pFWM007 | Plac::hupA-sbfp2::aph frt (KanR, AmpR) | This work |
| pFWB006 | pDEST::hupA-tagBFP::aph frt (KanR, AmpR) | This work |
| pBVS32 | pDEST::leuB’-Pj23100::tagRFP-T::aph frt-leuB” (KanR, AmpR) | This work |
| pEcTagRFP657 | E. coli codon-optimized TagRFP657 gene | This work |
| pERB004 | pERA001::leuB’-Pj23100::tagRFP657::aph frt-leuB” (KanR, TetR) | This work |
| pERB005 | pERA001::Pt7::tagRFP657::aph frt (KanR,TetR) | This work |
| pFWB009 | pDEST::hupA-tagRFP-T::aph frt (KanR, AmpR) | This work |
| pFWM006 | Plac::hupA-mKO2::aph frt (KanR, AmpR) | This work |
| pFWB019 | pDEST::hupA-mKate2::aph frt (KanR, AmpR) | This work |
| pBVS36 | pDEST:: TagYFP-MinDE::aph frt (KanR, AmpR) | This work |
| pBVS37 | pDEST:: TagGFP2-MinDE::aph frt (KanR, AmpR) | This work |
| pFWZ0 | Para::ftsZ::aph frt (KanR, CmR) | This work |
| pFWZ4 | Para::ftsZswtagRFP-T::aph frt (KanR, CmR) | This work |
| pFWZ5 | Para::ftsZswsfGFP::aph frt (KanR, CmR) | This work |
| pFWZ6 | Para::ftsZswtagCFP::aph frt (KanR, CmR) | This work |
| pFWZ7 | Para::ftsZswTC::aph frt (KanR, CmR) | This work |