Fig. 6.

Results of qRT-PCR analyses of putative upstream and downstream genes of ASKβ. Figures are grouped according to their putative functions or expression, as summarized in Table 1: BCP1 for cytoplasm degradation; DAF1, PCR11 and TIP5;1 for sperm cell development; ATTLP7 for two-celled pollen; ACA9 for aborted fertilization. Relative transcript levels in wild-type and ASKβ antisense transgenic lines are shown as follows: (A) TDF1 and MYB80; (B) BCP1; (C) DAF1, PCR11 and TIP5;1; (D) ATTLP6; and (E) ACA9. Expression levels were normalized to that of ACTIN7 and presented as the mean ± SD of three biologically independent experiments.