Table 1.
Compositional properties and spectroscopic data of apoA-I and apoA-4 in lipid-free and lipid-bound states
| (Initial molar ratio) | apoA-I | apoA-4 | ||||
|---|---|---|---|---|---|---|
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|
|
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| Lipid-free | rHDL (95:5:1) | rHDL (255:13:1) | Lipid-free | rHDL (95:5:1) | rHDL (255:13:1) | |
| WMF (nm) | 347 ± 1 | 344 ± 1 | 344 ± 1 | 351 ± 1 | 352 ± 1 | 351 ± 1 |
| α-helicity (%) | 55 ± 5 | 73 ± 4 | 41 ± 1 | 45 ± 1 | 47 ± 7 | 39 ± 11 |
| (α-helicity after glycation) | (27.5) | (37.9) | (41.1) | (30.6) | (19.5) | (28.0) |
| Size (Å) | 61–74 | 98 | 163, 184, 209 | 67 and 79 | 101, 107, 113 | 191, 227, 276 and 332 |
| Determind molar composition | ND | 95 ± 1:5:1 | 224 ± 4:13:1 | ND | 62 ± 7:5:1 | 200 ± 8:13:1 |
At calculation of α-helicity, protein concntration is 0.07 mg/ml at lipid-free state and 0.1 mg/ml at lipid-bound states Glycation condition; final 250 mM of fructose treat and incubation during 72 h at 5% CO2 containing incubator.