Figure 2.

Kyte & Doolittle Scale Mean Hydrophobicity Profile Analysis (A) Kyte & Doolittle Scale Mean Hydrophobicity Profile plot. Four hydrophobic regions are located at positions 60-132, 1990-2010, 2450-2500 and 3150-3300 of the dystrophin amino acid sequence (shown in the plot as marked with gray oval circles). Their corresponding peak values are 0.3, 0.15, 0.34 and 0.29. The third hydrophobic region has the highest peak value. (B) Involved hydrophobic regions and clinical type. A total of 715 cases (68.8%) have intact hydrophobic regions and there are 107/608 cases of DMD/BMD in this group. In contrast, when a hydrophobic region is involved in the mutation, the number of DMD/BMD cases is 135/188. The proportion of DMD (cases that do not meet the criteria of the reading-frame rule) in hydrophobic region-involved group (41.8%) is higher than the group in which hydrophobic regions are left intact (14.9%) (Χ²=88.09, p < 0.001). (C) DMD/BMD proportion in 4 hydrophobic regions. BMD cases are more common when hydrophobic region III is involved in the mutation (DMD/BMD=58/139). When any of the other hydrophobic regions are involved, DMD incidences are higher than BMD (DMD/BMD 58/40, 30/9 and 7/1 in hydrophobic regions I, II, IV, respectively)