Fig. 5.

CD4⁺ T-cells from n-3 PUFA-fed mice cultured in the presence of homologous HMS maintain the cholesterol content of lipid rafts. Splenic CD4⁺ T-cells were isolated from mice fed CO- or FO-containing diets for 2 weeks followed by membrane isolation (day 0) or after culture in the presence of either 5% FBS complete medium (FBS) or HMS (MS) for 5 days. Raft and soluble membrane fractions were isolated, and total phospholipid (PL) and cholesterol (chol) levels were expressed as a mol% ratio. Data represent means ± SEM; n = 3 replicates per diet group, and 10 mice were pooled per analysis. Letters indicate significant differences between culture conditions for the same membrane fraction and diet (P < 0.05).