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. 2015 Jun 18;4:e06938. doi: 10.7554/eLife.06938
Reagent Type Manufacturer Catalog # Comments
MMTV-PyMT+/tg; Postn+/− FVB male mouse Animal model Original lab n/a From original lab
MMTV-PyMT+/tg; Postn−/− FVB male mouse Animal model Original lab n/a From original lab
Postn−/− FVB female mice Animal model Original lab n/a From original lab
Postn+/+ FVB female mice Animal model Charles River Strain code: 207 Original was from France
PureGenome tissue DNA extraction kit Kit EMD Millipore 72635 Original not specified
MMTV-PYVT384 primer Nucleic acid Sequences provided by original authors; specific brand information will be left up to the discretion of the replicating lab and recorded later
MMTV-PYVT385 primer Nucleic acid
Postn-5′ primer Nucleic acid
Postn-3′ primer Nucleic acid
INT-as primer Nucleic acid
dNTPs (10 mM) Chemical Sigma–Aldrich D7295 Included during communication with authors. Original brand not specified
Taq-polymerase (with tubes of 10× PCR buffer and 25 mM MgCl2) Enzyme Sigma–Aldrich D4545 Included during communication with authors. Original brand not specified
PCR system Equipment Applied Biosystems StepOne Original not specified
Isoflurane Chemical Specific brand information will be left up to the discretion of the replicating lab and recorded later
StereoZoom stereomicroscope, zoom range 0.8×–4.0× Instrument Bausch & Lomb n/a Original a Leica M205 FA