MMTV-PyMT+/tg; Postn+/− FVB male mouse |
Animal model |
Original lab |
n/a |
From original lab |
MMTV-PyMT+/tg; Postn−/− FVB male mouse |
Animal model |
Original lab |
n/a |
From original lab |
Postn−/− FVB female mice |
Animal model |
Original lab |
n/a |
From original lab |
Postn+/+ FVB female mice |
Animal model |
Charles River |
Strain code: 207 |
Original was from France |
PureGenome tissue DNA extraction kit |
Kit |
EMD Millipore |
72635 |
Original not specified |
MMTV-PYVT384 primer |
Nucleic acid |
Sequences provided by original authors; specific brand information will be left up to the discretion of the replicating lab and recorded later |
MMTV-PYVT385 primer |
Nucleic acid |
Postn-5′ primer |
Nucleic acid |
Postn-3′ primer |
Nucleic acid |
INT-as primer |
Nucleic acid |
dNTPs (10 mM) |
Chemical |
Sigma–Aldrich |
D7295 |
Included during communication with authors. Original brand not specified |
Taq-polymerase (with tubes of 10× PCR buffer and 25 mM MgCl2) |
Enzyme |
Sigma–Aldrich |
D4545 |
Included during communication with authors. Original brand not specified |
PCR system |
Equipment |
Applied Biosystems StepOne |
|
Original not specified |
Isoflurane |
Chemical |
Specific brand information will be left up to the discretion of the replicating lab and recorded later |
StereoZoom stereomicroscope, zoom range 0.8×–4.0× |
Instrument |
Bausch & Lomb |
n/a |
Original a Leica M205 FA |