10× PCR buffer	1.50 µl
50 mM MgCl2	0.45 µl
10 mM dNTPs	0.30 µl
MMTV-PYVT384 primer	0.10 µl
MMTV-PYVT385 primer	0.10 µl
Taq-polymerase	0.20 µl
H2O	Bring up to 13 µl
DNA (1:20 dilution)	2 µl