Reagent	Type	Manufacturer	Catalog #	Comments
50 ml tubes	Labware	Sigma–Aldrich	CLS430290	Originally not specified
Hank's balanced salt solution (HBSS)	Buffer	Sigma–Aldrich	H6648	Included during communication with authors. Original brand not specified
Liberase TM	Enzyme	Roche	05401127001	–
Liberase TH	Enzyme	Roche	05401151001	–
DNase	Enzyme	Sigma–Aldrich	DN25	–
Phosphate buffered saline (PBS) without MgCl2 and CaCl2	Buffer	Sigma–Aldrich	D8537	Original brand not specified
EDTA	Chemical	Included during communication with authors. Specific brand information will be left up to the discretion of the replicating lab and recorded later
Bovine serum albumin (BSA)	Chemical	Sigma–Aldrich	A3803	Included during communication with authors. Original brand not specified
100 µm cell strainer	Labware	Corning	431752	Original brand not specified
2.5 ml syringe	Labware	Included during communication with authors. Specific brand information will be left up to the discretion of the replicating lab and recorded later
Fetal bovine serum (FBS)	Cell culture	Sigma–Aldrich	F0392	Original brand not specified
Polypropylene (opaque) FACS tubes	Labware	Specific brand information will be left up to the discretion of the replicating lab and recorded later
CD45 (clone 30-F11) PE-Cy5.5 antibody (rat IgG2b, kappa)	Antibodies	eBioscience	35-0451-80	Use at 1:300
CD31 (clone 390) Pac.Blue antibody (rat IgG2a, kappa)	Antibodies	Invitrogen	RM5228	Use at 1:200
CD34 (clone RAM34) PE antibody (rat IgG2a, kappa)	Antibodies	BD Pharmingen	551387	Use at 1:50
Rat IgG2b, kappa isotype control PE-Cy5.5	Antibodies	eBioscience	35-4031	Use at 1:300 dilution. Originally not specified
Rat IgG2a, kappa isotype control Pac.Blue	Antibodies	Invitrogen	R2a28	Use at 1:200 dilution. Originally not specified
Rat IgG2a, kappa isotype control PE	Antibodies	BD Pharmingen	553930	Use at 1:50 dilution. Originally not specified
7AAD	Chemical	BioLegend	420403	Use at 1:1000. Original brand not specified
Flow cytometric cell sorter	Instrument	BD Pharmingen	FACSAria II	Original from Beckman Coulter
FlowJo	Software	–	–	–
TRI reagent	Chemical	Sigma–Aldrich	T9424	Replaces RNA extraction kit from Qiagen
Oligo dT (18)	Nucleic acid	Life Technologies	SO132	Included during communication with authors. Original brand not specified
Oligo dT (23), Anchored	Nucleic acid	Sigma–Aldrich	O4387	Included during communication with authors. Original was Oligo dT (24). Original brand not specified
dNTPs (10 mM)	Chemical	Sigma–Aldrich	D7295	Included during communication with authors. Original brand not specified
25 mM MgCl2	Chemical	Sigma–Aldrich	M8787 (part of Sigma–Aldrich D4545 from Protocol 1)	Included during communication with authors. Original brand not specified
Superscript II (with tube of 5× buffer and 100 mM DTT)	Enzyme	Life Technologies	18064-014	Included during communication with authors
RNase inhibitor	Enzyme	Sigma–Aldrich	R1274	Included during communication with authors. Original was RNasin
POSTN 5′ primer	Nucleic acid	Sequences provided by original authors; specific brand information will be left up to the discretion of the replicating lab and recorded later
POSTN 3′ primer	Nucleic acid
GAPDH 5′ primer	Nucleic acid
GAPDH 3′ primer	Nucleic acid
Power SYBR green PCR master mix	Buffer	Life Technologies	4368577	–
Real-time PCR system	Equipment	Applied Biosystems StepOne	–	Original was from Roche or a StepOnePlus from Applied Biosystems