Forward primer	1.0 µl of 5 µM (5 pmol/µl)
Reverse primer	1.0 µl of 5 µM (5 pmol/µl)
MgCl2	0.4 µl (for 2 mM)
H2O	Bring up to 4.0 µl
SYBR mix	1.0 µl