Fig. 5.

Change of related signal proteins in the cytoprotective effect of Wnt3a-CM in Sh-SY5Y treated with 6-OHDA. SH-SY5Y cells were treated with vehicle, 6-OHDA (100 μM), Ctrl-CM, or Wnt3a-CM (40 %) 20 min prior to 6-OHDA for 24 h, the protein levels of 2 phosphorylation forms of GSK3β and β-catenin (a) were detected by Western blot with β-actin as internal control. The band intensities were measured by Quantity One software and normalized to the expression of β-actin in SHSY5Y cells. The ratio of p-GSK3β (Tyr216) to p-GSK3β (Ser9) (b) and the relative levels of β-catenin (c) were expressed in histogram. Data were presented as mean ± SD of 3 experiments. *P < 0.05 compared to the control, †P < 0.05 compared to Ctrl-CM + 6-OHDA group